Recombinant Mouse Arrb1 protein, His-tagged

• Cat.No.: ARRB1-1857M
• Product Overview: Recombinant Arrb1 protein (Pro182-Glu359), fused to His-tag at N-terminus,, was expressed in E.coli.

Specification

• Description: Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described.
• Source: E.coli
• Species: Mouse
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Protein length: 178
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Arrb1
• Official Symbol: Arrb1
• Synonyms: ARB1; ARR1; ARR-B1; Arrestin 2
• Gene ID: 109689
• mRNA Refseq: NM_177231.2
• Protein Refseq: NP_796205.1
• UniProt ID: Q8BWG8

Reviews

11/17/2022

It demonstrates excellent performance in ELISA, consistently delivering accurate and reliable results.

08/20/2017

I highly recommend the ARRB1 protein for a variety of research applications.

10/02/2016

Great performance in ELISA.

Q&As

What are the potential therapeutic implications of targeting ARRB1?

Targeting ARRB1 or its associated signaling pathways holds potential therapeutic implications for various diseases. Since ARRB1 is involved in regulating GPCR signaling, modulating its activity may provide a means to selectively enhance or reduce the signaling of specific GPCRs. This could be relevant in the treatment of diseases where dysregulated GPCR signaling is implicated, such as certain types of cancer, neurodegenerative disorders, and cardiovascular diseases.

What are the implications of ARRB1 malfunction or dysregulation?

Dysregulation of ARRB1 can lead to altered GPCR signaling and contribute to various diseases, including cardiovascular disorders, cancer, and neurological disorders.

In which tissues or organs is ARRB1 expressed?

ARRB1 is expressed in a wide range of tissues and organs, including the brain, heart, liver, kidneys, lungs, and immune cells.

How does ARRB1 interact with GPCRs?

ARRB1 binds to phosphorylated GPCRs, which occurs when GPCRs are activated by ligand binding. This binding helps recruit other proteins involved in the desensitization and internalization of the receptor.

Can ARRB1 be targeted for therapeutic interventions?

ARRB1 has emerged as a potential target for drug development due to its involvement in various diseases. Modulating ARRB1 activity could offer opportunities for developing novel therapeutics aiming to fine-tune GPCR signaling and related pathways.

Are there any known interacting proteins or partners of ARRB1?

ARRB1 interacts with various other proteins, including clathrin, which facilitates receptor endocytosis, and signaling molecules such as kinases and phosphatases, which mediate downstream signaling events.

Are there any other functions or roles of ARRB1?

In addition to its role in GPCR regulation, ARRB1 has been implicated in intracellular signaling pathways, including mitogen-activated protein kinase (MAPK) signaling, cell migration, and regulation of gene transcription.