Recombinant Mouse ARSA protein(Met1-Ser506), His-tagged

• Cat.No.: Arsa-2334M
• Product Overview: Recombinant Mouse ARSA (NP_033843.2)(Met 1-Ser 506) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ser506
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse ARSA consists of 500 amino acids and has a calculated molecular mass of 53.5 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Arsa arylsulfatase A [ Mus musculus ]
• Official Symbol: ARSA
• Synonyms: ARSA; arylsulfatase A; cerebroside-sulfatase; ASA; As2; AS-A; As-2; TISP73; AW212749
• Gene ID: 11883
• mRNA Refseq: NM_009713
• Protein Refseq: NP_033843

Reviews

12/20/2021

By utilizing the ARSA protein, I can approach my research with unwavering confidence in its quality, along with the unparalleled support provided by its manufacturer.

03/05/2020

I am genuinely excited to embark on this scientific journey, empowered by the superior ARSA protein and the manufacturer's unwavering commitment to ensuring my success.

10/01/2017

The ARSA protein comes highly recommended due to its exceptional performance in ELISA assays.

Q&As

Are there any known genetic variations in the ARSA gene?

Yes, several genetic variations, including point mutations, deletions, and insertions, have been identified in the ARSA gene. These variations can lead to reduced ARSA activity and are responsible for different types and severities of metachromatic leukodystrophy.

Can ARSA deficiency be treated?

At present, there is no cure for metachromatic leukodystrophy caused by ARSA deficiency. However, there are some treatments available that aim to manage the symptoms and slow disease progression, such as enzyme replacement therapy and hematopoietic stem cell transplantation. Research is ongoing to develop more effective therapeutic strategies for this condition.

What happens if ARSA is deficient?

Deficiency of ARSA activity results in the accumulation of sulfatides within cells, particularly in the central and peripheral nervous systems. This buildup leads to the destruction of myelin, impairing nerve signal transmission and causing the symptoms associated with metachromatic leukodystrophy (MLD), such as motor and cognitive deterioration.

Is ARSA protein associated with any other diseases besides metachromatic leukodystrophy?

Yes, in addition to MLD, alterations in ARSA activity have been implicated in certain types of multiple sclerosis (MS). Although the exact relationship is not fully understood, it is believed that decreased ARSA function may contribute to the pathogenesis of certain forms of MS.