Recombinant Mouse Asah2, His tagged

• Cat.No.: Asah2-251M
• Product Overview: Recombinant Mouse Asah2 precursor Lumenal domain (Thr 34-Thr 756) (NP_061300.1), fused with a N-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Source: Human Cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The secreted recombinant mouse ASAH2 consists of 739 amino acids and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Asah2 N-acylsphingosine amidohydrolase 2 [ Mus musculus ]
• Official Symbol: Asah2
• Gene ID: 54447
• mRNA Refseq: NM_018830
• Protein Refseq: NP_061300

Reviews

04/04/2019

It reliably and accurately detects and quantifies target molecules, allowing for precise measurement of analytes in my samples.

02/25/2019

I highly recommend the ASAH2 Protein for its outstanding performance in ELISA assays.

11/22/2016

The sensitivity and specificity of the ASAH2 Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

Q&As

Can ASAH2 activity be regulated by post-translational modifications?

Post-translational modifications, such as phosphorylation, may impact ASAH2 activity, although further research is needed for a comprehensive understanding.

Are there any known inhibitors of ASAH2?

While specific inhibitors for ASAH2 are not yet available, researchers are actively exploring potential compounds that can modulate its activity.

In which cellular compartments is ASAH2 found?

ASAH2 is primarily localized in the lysosomes, where it carries out the ceramide hydrolysis process.

What are the consequences of ASAH2 deficiency?

Deficiency in ASAH2 activity can lead to the accumulation of ceramides, which may disrupt cellular signaling and contribute to disease development.

How is ASAH2 gene expression regulated?

ASAH2 gene expression can be regulated by various factors, including transcription factors, epigenetic modifications, and signaling pathways.

Are there any diseases associated with ASAH2?

Mutations in the ASAH2 gene have been associated with a rare form of hereditary sensory and autonomic neuropathy (HSAN) called HSAN1C.

Is ASAH2 ubiquitously expressed in all tissues?

Yes, ASAH2 is ubiquitously expressed in various tissues throughout the body.