Recombinant Mouse Bcam protein(Met1-Ala541), hFc-tagged

• Cat.No.: Bcam-6869M
• Product Overview: Recombinant Mouse BCAM (Q9R069) (Met 1-Ala 541) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Ala541
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HOS human osteogenic sarcoma cells. When cells are added to coated plates(5 μg/mL, 100μL/well), approximately 30-70% will adhere after 1 hour at 37°C.
• Molecular Mass: The secreted recombinant mouse BCAMFc is a disulfide-linked homodimer. The reduced monomer comprises 757 amino acids and has a calculated molecular mass of 84 kDa. As a result of glycosylation, the apparent molecular mass of rmBCAM/Fc monomer is approximately 95-105 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Bcam basal cell adhesion molecule [ Mus musculus ]
• Official Symbol: Bcam
• Synonyms: BCAM; basal cell adhesion molecule; lutheran antigen; Auberger b antigen; Lutheran blood group; lutheran glycoprotein; B-CAM cell surface glycoprotein; Lu; Gplu; B-CAM; 1200005K12Rik
• Gene ID: 57278
• mRNA Refseq: NM_020486
• Protein Refseq: NP_065232