| Description : |
This gene encodes a member of the Bcl-2 family of apoptosis regulators. The encoded protein is localized to the inner and outer mitochondrial membranes and regulates the programmed cell death pathway during development and tissue homeostasis. This protein binds to voltage-dependent anion channels in the outer mitochondrial membrane to facilitate the uptake of calcium ions. Mice embryos lacking this gene survived for two weeks and exhibited cell death of immature hematopoietic cells and neurons. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
| Source : |
E.coli |
| Species : |
Mouse |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, 5 % Trehalose. |
| Molecular Mass : |
Approximately 23.7 kDa, a single non-glycosylated polypeptide chain containing 211 amino acids. |
| Protein length : |
211 |
| AA Sequence : |
SQSNRELVVDFLSYKLSQKGYSWSQ FSDVEENRTEAPEETEAERETPSAI NGNPSWHLADSPAVNGATGHSSSLD AREVIPMAAVKQALREAGDEFELRY RRAFSDLTSQLHITPGTAYQSFEQV VNELFRDGVNWGRIVAFFSFGGALC VESVDKEMQVLVSRIASWMATYLND HLEPWIQENGGWDTFVDLYGNNAAA ESRKGQERFNR |
| Endotoxin : |
Less than 0.1 EU/µg of rMuBcl-xL as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
