Recombinant Mouse BECN1 Protein (1-448 aa), His-SUMO-tagged

• Cat.No.: BECN1-2059M
• Product Overview: Recombinant Mouse BECN1 Protein (1-448 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity).
• Source: E. coli
• Species: Mouse
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 67.6 kDa
• Protein length: 1-448 aa
• AA Sequence: MEGSKASSSTMQVSFVCQRCSQPLK LDTSFKILDRVTIQELTAPLLTTAQ AKPGETQEEEANSGEEPFIETRQDG VSRRFIPPARMMSTESANSFTLIGE ASDGGTMENLSRRLKVTGDLFDIMS GQTDVDHPLCEECTDTLLDQLDTQL NVTENECQNYKRCLEILEQMNEDDS EQLQRELKELALEEERLIQELEDVE KNRKVVAENLEKVQAEAERLDQEEA QYQREYSEFKRQQLELDDELKSVEN QVRYAQIQLDKLKKTNVFNATFHIW HSGQFGTINNFRLGRLPSVPVEWNE INAAWGQTVLLLHALANKMGLKFQR YRLVPYGNHSYLESLTDKSKELPLY CSGGLRFFWDNKFDHAMVAFLDCVQ QFKEEVEKGETRFCLPYRMDVEKGK IEDTGGSGGSYSIKTQFNSEEQWTK ALKFMLTNLKWGLAWVSSQFYNK
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: Becn1 beclin 1, autophagy related [ Mus musculus ]
• Official Symbol: BECN1
• Synonyms: BECN1; Atg6; MGC6843; 4921513J16Rik; 5430417M23Rik
• Gene ID: 56208
• mRNA Refseq: NM_019584
• Protein Refseq: NP_062530
• UniProt ID: O88597