Recombinant Mouse Col2a1 Protein, His-tagged

• Cat.No.: Col2a1-1279M
• Product Overview: Recombinant Mouse Col2a1 Protein (Asp1242-Leu1487) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes the alpha-1 subunit of the fibril-forming type II collagen, the major component of cartilage and the vitreous humor of the eye. The encoded preproprotein forms homotrimeric, triple helical procollagen that undergoes proteolytic processing during fibirl formation. Mice harboring certain mutations in this gene exhibit severe chondrodysplasia characterized by short limbs and trunch, craniofacial deformities and cleft palate. A complete lack of the encoded protein in mice results in postnatal lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.2 kDa
  Accurate Molecular Mass: 35 kDa
• Protein length: Asp1242-Leu1487
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Col2a1 collagen, type II, alpha 1 [ Mus musculus (house mouse) ]
• Official Symbol: Col2a1
• Synonyms: Col2a1; collagen, type II, alpha 1; Dmm; Lpk; Col2; Del1; Col2a; Col2a-1; M100413; Rgsc413; Rgsc856; collagen alpha-1(II) chain; alpha-1 type II collagen; chondrocalcin; procollagen, type II, alpha 1
• Gene ID: 12824
• mRNA Refseq: NM_031163
• Protein Refseq: NP_112440
• UniProt ID: P28481