Recombinant Mouse Defa5 Protein, His&GST-tagged

• Cat.No.: Defa5-1328M
• Product Overview: Recombinant Mouse Defa5 Protein (Asp20-Ser93) with N-His&GST tag was expressed in E. coli.

Specification

• Description: Predicted to enable protein homodimerization activity. Acts upstream of or within defense response to bacterium. Predicted to be located in intracellular membrane-bounded organelle and midbody. Predicted to be active in extracellular space. Is expressed in ileum. Orthologous to several human genes including DEFA1B (defensin alpha 1B); DEFA3 (defensin alpha 3); and DEFA5 (defensin alpha 5).
• Source: E. coli
• Species: Mouse
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 38.4 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Asp20-Ser93
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Defa5 defensin, alpha, 5 [ Mus musculus (house mouse) ]
• Official Symbol: Defa5
• Synonyms: DEFA5; defensin, alpha, 5; alpha-defensin 5; defensin, alpha, 29; defensin-related cryptdin-5; Defa29; Defcr5; Defcr29
• Gene ID: 13239
• mRNA Refseq: NM_007851
• Protein Refseq: NP_031877
• UniProt ID: P28312

Reviews

08/16/2019

The product's established reputation in the scientific community provided assurance of its reliability.

07/07/2019

The product's innovative features hinted at its potential for advancing our research in uncharted territories.

12/20/2016

We were excited about the product's role in driving future discoveries in our field of study.

Q&As

Is DEFA5 linked to any diseases?

Altered DEFA5 expression has been associated with various gastrointestinal disorders, including inflammatory bowel diseases (IBD).

Are there specific diseases where DEFA5 is implicated?

DEFA5 has been implicated in conditions such as inflammatory bowel diseases (IBD) and other gastrointestinal disorders.

What is the significance of DEFA5?

DEFA5, known as Defensin Alpha 5, is a crucial member of the defensin family, functioning as an antimicrobial peptide.

What role does DEFA5 play in innate immunity?

DEFA5 contributes to innate immunity by directly targeting and inhibiting the growth of pathogenic microorganisms in the gut.

How does DEFA5 function in host defense?

DEFA5 acts as an antimicrobial peptide, defending the gut against bacterial infections and helping maintain a balanced gut microbiota.

Can DEFA5 be a potential therapeutic target?

DEFA5 is being investigated as a potential therapeutic target for conditions involving gut infections and imbalances in the gut microbiota.

What is the primary tissue of expression for DEFA5?

DEFA5 is primarily expressed in Paneth cells located in the small intestine, contributing to innate immune defense in the gut.