Recombinant Mouse Dtd2 Protein, Myc/DDK-tagged
Cat.No. : | Dtd2-2668M |
Product Overview : | Purified recombinant protein of mouse full-length D-tyrosyl-tRNA deacylase 2 (Dtd2), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
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Description : | Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation. |
Source : | HEK293T |
Species : | Mouse |
Tag : | Myc/DDK |
Molecular Mass : | 18.2 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | Dtd2 D-tyrosyl-tRNA deacylase 2 [ Mus musculus (house mouse) ] |
Official Symbol : | Dtd2 |
Synonyms : | Dtd2; D-tyrosyl-tRNA deacylase 2; ATD; 4930578F06Rik; 6530401N04Rik; B830049N13Rik; D-aminoacyl-tRNA deacylase 2; L-alanyl-tRNA deacylase; animalia-specifc tRNA deacylase; animalia-specific tRNA deacylase; probable D-tyrosyl-tRNA(Tyr) deacylase 2; putative D-tyrosyl-tRNA deacylase 2; putative D-tyrosyl-tRNA(Tyr) deacylase 2; EC 3.1.1.96 |
Gene ID : | 328092 |
mRNA Refseq : | NM_029545 |
Protein Refseq : | NP_083821 |
UniProt ID : | Q8BHA3 |
Products Types
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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