Recombinant Mouse Efna4 protein(Met1-Gly176), hFc-tagged

• Cat.No.: Efna4-4056M
• Product Overview: Recombinant Mouse EFNA4 (NP_031936.2) without the pro peptide (Met 1-Gly 176) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Gly176
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. 1. Immobilized human EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml. 2. Immobilized mouse EphA7 at 2 μg/ml (100 μl/well) can bind mouse EphrinA4 Fc Chimera with a linear range of 0.08-10 ng/ml.
• Molecular Mass: The recombinant mouse EFNA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 392 amino acids and has a predicted molecular mass of 43.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA4/Fc monomer is approximately 52 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efna4 ephrin A4 [ Mus musculus ]
• Official Symbol: Efna4
• Synonyms: EFNA4; ephrin A4; ephrin-A4; EPH-related receptor tyrosine kinase ligand 4; Epl4; EFL-4; LERK-4
• Gene ID: 13639
• mRNA Refseq: NM_007910
• Protein Refseq: NP_031936