Recombinant Mouse Efnb1 protein(Met1-Ser229), His-tagged

• Cat.No.: Efnb1-4050M
• Product Overview: Recombinant Mouse EFNB1 (NP_034240.1) (Met 1-Ser 229) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ser229
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to compete with mouse EphrinB1 / Fc for binding to immobilized mouse EphB3 in a functional ELISA assay.
• Molecular Mass: The secreted recombinant mouse EFNB1 consists of 211 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNB1 is approximately 33-38 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb1 ephrin B1 [ Mus musculus ]
• Official Symbol: Efnb1
• Synonyms: EFNB1; ephrin B1; ephrin-B1; Cek ligand; ELK ligand; Cek5 ligand; CEK5 receptor ligand; stimulated by retinoic acid gene 1 protein; EPH-related receptor tyrosine kinase ligand 2; Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2
• Gene ID: 13641
• mRNA Refseq: NM_010110
• Protein Refseq: NP_034240