Recombinant Mouse Epor protein(Met1-Pro249), hFc-tagged

• Cat.No.: Epor-499M
• Product Overview: Recombinant Mouse EPOR (NP_034279.3) (Met1-Pro249) was expressed in Insect Cells, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Pro249
• Form: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.05-0.2 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse EPO-His at 10μg/mL (100 μL/well) can bind mouse EPOR-Fc. The EC50 of mouse EPOR-Fc is 0.06-0.13μg/mL.
• Molecular Mass: The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epor erythropoietin receptor [ Mus musculus ]
• Official Symbol: Epor
• Synonyms: EPOR; erythropoietin receptor; EPO-R
• Gene ID: 13857
• mRNA Refseq: NM_010149
• Protein Refseq: NP_034279