Recombinant Mouse F12 protein, His & T7-tagged

• Cat.No.: F12-774M
• Product Overview: Recombinant Mouse F12 aa. (Phe352~Tyr587 (Accession # Q80YC5)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes a glycoprotein coagulation factor that plays an important role in the intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that is autoactivated upon contact with negatively charged surfaces or misfolded protein aggregates. Mice lacking the encoded protein have a severe defect in forming stable fibrin clots.
• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.1kDa
• Protein length: Phe352~Tyr587 (Accession # Q80YC5)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Mus musculus (house mouse) ]
• Official Symbol: F12
• Synonyms: HAF; FXII; factor XII; coagulation factor XII; hageman factor
• Gene ID: 58992
• mRNA Refseq: NM_021489.3
• Protein Refseq: NP_067464.2
• UniProt ID: Q80YC5