Recombinant Mouse Gfra1 protein(Met1-Ser425), His-tagged

• Cat.No.: Gfra1-722M
• Product Overview: Recombinant Mouse Gfra1 (AAB86600.1)(Met 1-Ser 425) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ser425
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF. 2. Measured by its ability to bind with human GDNF in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse GFRα1consists of 412 amino acids and has a calculated molecular mass of 46.3 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gfra1 glial cell line derived neurotrophic factor family receptor alpha 1 [ Mus musculus ]
• Official Symbol: Gfra1
• Synonyms: GFRA1; glial cell line derived neurotrophic factor family receptor alpha 1; GDNF family receptor alpha-1; GDNFR-alpha; GFR alpha-1; GFR-alpha-1; GDNFR-alpha-1; GDNF receptor alpha-1; TGF-beta-related neurotrophic factor receptor 1; AU042498
• Gene ID: 14585
• mRNA Refseq: NM_010279
• Protein Refseq: NP_034409