Recombinant Mouse Gid4 Protein, Myc/DDK-tagged

• Cat.No.: Gid4-3205M
• Product Overview: Purified recombinant protein of mouse full-length GID complex subunit 4, VID24 homolog (Gid4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Substrate-recognition subunit of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Binds proteins and peptides with a Pro/N-degron consisting of an unmodified N-terminal Pro followed by a small residue, and has the highest affinity for the peptide Pro-Gly-Leu-Trp. Binds peptides with an N-terminal sequence of the type Pro-[Ala,Gly]-[Leu,Met,Gln,Ser,Tyr]-[Glu,Gly,His,Ser,Val,Trp,Tyr]. Does not bind peptides with an acetylated N-terminal Pro residue.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 24.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gid4 GID complex subunit 4, VID24 homolog [ Mus musculus (house mouse) ]
• Official Symbol: Gid4
• Synonyms: Gid4; GID complex subunit 4, VID24 homolog; 4933439F18Rik; glucose-induced degradation protein 4 homolog; vacuolar import and degradation protein 24 homolog
• Gene ID: 66771
• mRNA Refseq: NM_025757
• Protein Refseq: NP_080033
• UniProt ID: Q9CPY6