Recombinant Mouse GLO1 protein(Ala2-Ile184), His-tagged

• Cat.No.: Glo1-872M
• Product Overview: Recombinant Mouse GLO1 (NP_079650.3) (Ala 2-Ile 184) was expressed in E. coli, with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: N-His
• Protein length: Ala2-Ile184
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse GLO1 consisting of 190 amino acids and has a calculated molecular mass of 21.6 kDa. The apparent molecular mass of the rmGOL1 is approximately 25 and 48 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer and dimer respectively.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Glo1 glyoxylase 1 complex [ Mus musculus ]
• Official Symbol: GLO1
• Synonyms: GLO1; glyoxylase 1 complex; Glo-1
• Gene ID: 14648

Reviews

06/28/2022

Timely protein expression optimization; improves our research efficiency.

07/20/2020

Accurate protein structural analysis; crucial for our studies.

11/05/2019

Dependable antibody validation; boosts our immunoprecipitation studies.

Q&As

What role does GLO1 play in the pathogenesis of diabetes and related metabolic disorders?

GLO1 is implicated in diabetes and metabolic disorders, influencing glycation end products and insulin signaling.

What are the mechanisms by which GLO1 modulates cancer cell proliferation and metastasis?

GLO1 modulates cancer cell growth and spread, affecting tumor development and metastasis.

How does GLO1 interact with other components of the glyoxalase system in maintaining cellular homeostasis?

GLO1 works with the glyoxalase system to regulate cellular detoxification and protect against metabolic stress.

How does GLO1 expression influence cellular oxidative stress and aging?

GLO1 expression affects cellular oxidative stress, playing a role in aging and age-related cellular damage.

How do genetic variations in the GLO1 gene affect its enzymatic activity and the susceptibility to chronic diseases?

Genetic variations in GLO1 alter its activity, affecting disease risk and response to metabolic stress.

What is the impact of GLO1 overexpression or deficiency on neurological functions and neurodegenerative diseases?

Changes in GLO1 levels impact neurological function and may contribute to the progression of neurodegenerative diseases.

How does GLO1 regulate the detoxification of methylglyoxal and other reactive aldehydes in cells?

GLO1 detoxifies methylglyoxal and other aldehydes, preventing cellular damage and maintaining metabolic balance.