Recombinant Mouse HSPA8 Protein, His-tagged

• Cat.No.: HSPA8-49M
• Product Overview: Recombinant Mouse HSPA8 Protein, fused to His-tag, was expressed in E. coli.

Specification

• Description: Enables ATP hydrolysis activity; phosphatidylserine binding activity; and unfolded protein binding activity. Involved in several processes, including chaperone-mediated protein folding; late endosomal microautophagy; and positive regulation by host of viral genome replication. Acts upstream of or within several processes, including chaperone cofactor-dependent protein refolding; clathrin coat disassembly; and positive regulation of mRNA splicing, via spliceosome. Located in several cellular components, including extracellular exosome; glycinergic synapse; and photoreceptor ribbon synapse. Is active in presynapse. Is expressed in several structures, including 1st branchial arch mandibular component; alimentary system; brain; eye; and forelimb long bone. Human ortholog(s) of this gene implicated in Parkinson's disease and renal hypertension. Orthologous to human HSPA8 (heat shock protein family A (Hsp70) member 8).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Supplied as a 0.2 μm filtered solution in PBS, pH8.0.
• Molecular Mass: ~74 kDa
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMSKGP AVGIDLGTTYSCVGVFQHGKVEIIA NDQGNRTTPSYVAFTDTERLIGDAA KNQVAMNPTNTVFDAKRLIGRRFDD AVVQSDMKHWPFMVVNDAGRPKVQV EYKGETKSFYPEEVSSMVLTKMKEI AEAYLGKTVTNAVVTVPAYFNDSQR QATKDAGTIAGLNVLRIINEPTAAA IAYGLDKKVGAERNVLIFDLGGGTF DVSILTIEDGIFEVKSTAGDTHLGG EDFDNRMVNHFIAEFKRKHKKDISE NKRAVRRLRTACERAKRTLSSSTQA SIEIDSLYEGIDFYTSITRARFEEL NADLFRGTLDPVEKALRDAKLDKSQ IHDIVLVGGSTRIPKIQKLLQDFFN GKELNKSINPDEAVAYGAAVQAAIL SGDKSENVQDLLLLDVTPLSLGIET AGGVMTVLIKRNTTIPTKQTQTFTT YSDNQPGVLIQVYEGERAMTKDNNL LGKFELTGIPPAPRGVPQIEVTFDI DANGILNVSAVDKSTGKENKITITN DKGRLSKEDIERMVQEAEKYKAEDE KQRDKVSSKNSLESYAFNMKATVED EKLQGKINDEDKQKILDKCNEIISW LDKNQTAEKEEFEHQQKELEKVCNP IITKLYQSAGGMPGGMPGGFPGGGA PPSGGASSGPTIEEVDLELEHHHHH H
• Purity: >90%
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.25 mg/mL

Gene Information

• Gene Name: Hspa8 heat shock protein 8 [ Mus musculus (house mouse) ]
• Official Symbol: HSPA8
• Synonyms: Hsc70; Hsc71; Hsc73; Hsp73; Hspa10; 2410008N15Rik
• Gene ID: 15481
• mRNA Refseq: NM_031165
• Protein Refseq: NP_112442
• UniProt ID: P63017

Reviews

12/01/2021

The lab product's precision and quality are noteworthy.

10/25/2020

The customer service team was accommodating, and the delivery exceeded my expectations.

05/10/2018

This lab product is synonymous with excellence.

Q&As

In the context of cellular stress, how does HSPA8 contribute to the heat shock response, and what downstream signaling pathways does it modulate to promote cell survival?

HSPA8 is a central player in the heat shock response, assisting in the refolding of denatured proteins. It modulates downstream pathways such as HSF1 activation, orchestrating a cellular survival response.

Can you discuss the involvement of HSPA8 in neurodegenerative diseases, and how does its chaperone activity influence protein aggregation and neuronal dysfunction?

HSPA8 plays a role in neurodegenerative diseases, mitigating protein aggregation and neuronal dysfunction. Its chaperone activity is crucial for maintaining protein homeostasis in the nervous system.

How does HSPA8 participate in the regulation of apoptosis, and what anti-apoptotic mechanisms does it employ to maintain cellular viability under stress conditions?

HSPA8 exhibits anti-apoptotic functions by interacting with pro-apoptotic proteins and preventing their activation. Its role in apoptosis regulation contributes to cell survival during stress.

What is the significance of HSPA8 in autophagy, and how does it influence the selective degradation of misfolded proteins and damaged organelles?

HSPA8 is involved in autophagy, facilitating the clearance of misfolded proteins and damaged organelles. It interacts with autophagy receptors, promoting their recognition by autophagosomes.

What are the mechanisms by which HSPA8 regulates the immune response, particularly its involvement in antigen presentation and the modulation of immune cell functions?

HSPA8 participates in antigen presentation and modulates immune cell functions. It chaperones antigens for presentation on major histocompatibility complexes, influencing immune recognition and response.