Recombinant Mouse Htt protein, His & GST-tagged

• Cat.No.: Htt-1597M
• Product Overview: Recombinant Mouse Htt aa. (Thr782~Phe920) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Thr782~Phe920
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >92%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 6.5

Gene Information

• Gene Name: Htt huntingtin [ Mus musculus (house mouse) ]
• Official Symbol: Htt
• Synonyms: Htt; huntingtin; Hd; Hdh; IT15; AI256365; C430023I11Rik; HD protein homolog; Huntington disease gene homolog; huntington disease protein homolog
• Gene ID: 15194
• mRNA Refseq: NM_010414.3
• Protein Refseq: NP_034544.1
• UniProt ID: G3X9H5

Reviews

06/24/2021

This protein synthesis process is efficient and reliable, reducing experimental time and cost.

02/20/2021

This product has strict quality control and complies with relevant industry standards.

09/08/2020

When experiments were performed with this protein, the sensitivity and accuracy of the experiments were significantly improved.

Q&As

What diseases is HTT associated with?

HTT is associated with neurodegenerative diseases such as Huntington's disease, Parkinson's disease, and Alzheimer's disease.

How can animal models be used to study the function and disease mechanism of HTT protein?

Animal models carrying human mutant genes can be constructed using transgenic or gene editing technology to study the role of HTT protein in vivo and disease mechanisms. These animal models can be used to study the effects of HTT proteins on neuronal function, synaptic transmission, behavior, and more, as well as to evaluate the effects of potential therapeutic strategies.

What are the therapeutic strategies for HTT protein?

Therapeutic strategies for HTT protein include inhibiting its expression, reducing its activity, or changing its modification state. A number of small molecule inhibitors and gene therapy strategies targeting HTT proteins are currently being studied.

Is there a challenge in HTT protein research?

There are still some challenges in the research of HTT proteins, such as the need to better understand their structure and functional relationships, and develop more effective drugs or treatments. In addition, due to the complexity and individual differences of neurodegenerative diseases, therapeutic strategies targeting HTT proteins also need to be adapted and optimized according to individual conditions.

How does HTT protein interact with other proteins or signaling pathways?

HTT protein can interact with other proteins and signaling pathways. For example, HTT proteins can interact with transcription factors to affect gene transcription, and can also interact with signaling pathways such as MAPK (mitogen-activated protein kinase) to participate in cell proliferation and apoptosis.

How can HTT protein be used for disease diagnosis?

By measuring the expression level and modification status of HTT protein, it can be used to diagnose diseases such as Huntington's disease. At present, there are some detection methods for HTT protein, such as western blotting, immunohistochemistry, etc.