Recombinant Mouse Insulin-like Growth Factor Binding Protein 3

• Cat.No.: Igfbp3-1750M
• Product Overview: Recombinant Mouse Igfbp3 was fused to the human CD33 signal peptide. This chimeric protein was expressed inNSO cells.

Specification

• Cat. No.: Igfbp3-1750M
• Description: Insulin-like growth factor binding protein 3, also known as IGFBP3, is a member of the insulin-like growth factor binding protein (IGFBP) family that are cysteine-rich proteins that act as a carrier protein. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.
• Molecular Weight: The predicted molecular weight of Recombinant Mouse IGFBP-3 is Mr 29;.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40-50 kDa.
• Source: Mammalian cells
• State Of Matter: Lyophilized.
• Purity: >95% by SDS Page and analyzed by silver stain.
• Formulation: This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Mouse IGFBP-3 was determined by its ability to inhibit the biological activity of rmIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The expected ED50 for this effect is typically 0.125 - 0.5 μg/ml in the presence of 30 ng/ml rmIGF-II.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20℃ to -70℃. After aseptic reconstitution, this protein may be stored at 2℃ to 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Igfbp3 insulin-like growth factor binding protein 3 [ Mus musculus ]
• Synonyms: Igfbp3; insulin-like growth factor binding protein 3; IGFBP-3; IGgfbp3; AI649005; OTTMUSP00000005343
• Gene ID: 16009
• mRNA Refseq: NM_008343
• Protein Refseq: NP_032369
• UniProt ID: P47878
• Chromosome Location: 11 A1; 11 1.35 cM
• Pathway: p53 signaling pathway
• Function: growth factor binding; insulin-like growth factor binding

Reviews

05/04/2020

A manufacturer that offers customization options can enable the researcher to adhere to their experimental design requirements more effectively.

01/20/2019

A reliable manufacturer should be able to provide a consistently pure and high-quality IGFBP3 protein, ensuring that the samples are suitable for research purposes.

01/02/2019

This can help ensure compatibility between different components used in the trials and ease the overall experimental workflow.

Q&As

Are there any genetic factors that influence IGFBP3 protein levels?

Yes, genetic variations can affect IGFBP3 expression, potentially leading to differences in protein levels among individuals.

How does IGFBP3 protein influence bone health?

IGFBP3 is involved in bone metabolism and is associated with bone mineral density. Low levels of IGFBP3 have been linked to decreased bone density and increased risk of fractures.

Are there any side effects associated with therapies targeting IGFBP3?

The potential side effects of therapies targeting IGFBP3 are still being studied, and it varies depending on the specific treatment approach being used.

How do lifestyle factors such as diet and exercise influence IGFBP3 levels?

Healthy lifestyle choices, including a balanced diet and regular exercise, can positively influence IGFBP3 levels, promoting overall health and reducing the risk of related diseases.

What are the implications of IGFBP3 deficiency in children's growth and development?

IGFBP3 deficiency can impact children's growth and development, potentially leading to short stature and delayed puberty, as IGFBP3 is a key regulator of growth hormone actions.