Recombinant Mouse Itfg2 Protein, MYC/DDK-tagged

• Cat.No.: Itfg2-172M
• Product Overview: Purified recombinant protein of full-length mouse integrin alpha FG-GAP repeat containing 2 (Itfg2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 49 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Itfg2 integrin alpha FG-GAP repeat containing 2 [ Mus musculus (house mouse) ]
• Official Symbol: Itfg2
• Synonyms: Itfg2; integrin alpha FG-GAP repeat containing 2; AI646725; 2700050P07Rik; KICSTOR complex protein ITFG2; integrin-alpha FG-GAP repeat-containing protein 2
• Gene ID: 101142
• mRNA Refseq: NM_133927
• Protein Refseq: NP_598688
• UniProt ID: Q91WI7