Recombinant Mouse MET protein(Met1-Asn929), His-tagged

• Cat.No.: MET-1139M
• Product Overview: Recombinant Mouse MET (EDL13881.1) extracellular domain (Met 1-Asn 929) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Asn929
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized HGF Protein, Mouse, Recombinant at 2 μg/mL (100 μL/well) can bind c-MET Protein, Mouse, Recombinant (ECD, His Tag), the EC50 is 80-400 ng/mL.
• Molecular Mass: The recombinant mouse Met is a heterodimer composed of the proteolytically cleaved α and β subunits. The α and β heterodimer consists of 916 amino acids and has a predicted molecular mass of 102 (α =32 + β=70) kDa. The apparent molecular mass of the rmMET heterodimer thus is approximately 41.8 kDa and 87.2 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Met met proto-oncogene [ Mus musculus ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; HGF; HGFR; Par4; c-Met; AI838057
• Gene ID: 17295
• mRNA Refseq: NM_008591
• Protein Refseq: NP_032617