Recombinant Mouse Myh6 Protein, His-tagged

• Cat.No.: Myh6-2468M
• Product Overview: Recombinant Mouse Myh6 Protein (Glu857-Ile1117) with a N-His tag was expressed in E. coli.

Specification

• Description: Enables microfilament motor activity. Involved in cardiac muscle contraction. Acts upstream of or within several processes, including adult heart development; muscle cell development; and regulation of heart contraction. Located in Z disc and stress fiber. Part of myosin complex. Is expressed in several structures, including brown fat; embryo mesenchyme; great vessel of heart; heart; and skeletal musculature. Used to study dilated cardiomyopathy; dilated cardiomyopathy 1EE; and hypertrophic cardiomyopathy 14. Human ortholog(s) of this gene implicated in atrial heart septal defect (multiple); heart conduction disease (multiple); and intrinsic cardiomyopathy (multiple). Orthologous to human MYH6 (myosin heavy chain 6).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 31.7 kDa
  Accurate Molecular Mass: 37 kDa
• Protein length: Glu857-Ile1117
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Myh6 myosin, heavy polypeptide 6, cardiac muscle, alpha [ Mus musculus (house mouse) ]
• Official Symbol: Myh6
• Synonyms: Myh6; myosin, heavy polypeptide 6, cardiac muscle, alpha; Myhca; Myhc-a; AA517445; alphaMHC; alpha-MHC; A830009F23Rik; myosin-6
  alpha cardiac MHC
  alpha myosin
  cardiomyopathy, hypertrophic 1
  myHC-alpha
  myosin heavy chain 6
  myosin heavy chain, cardiac muscle, adult
• Gene ID: 17888
• mRNA Refseq: NM_010856
• Protein Refseq: NP_034986
• UniProt ID: Q02566