Recombinant Mouse NEK3 protein(Met1-Ala509), His&GST-tagged

• Cat.No.: NEK3-924M
• Product Overview: Recombinant Mouse NEK3 (ABK42288.1) (Met 1-Ala 509) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: N-His&GST
• Protein length: Met1-Ala509
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
• Bio-activity: The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.
• Molecular Mass: The secreted recombinant mouse NEK3/GST chimera consists of 746 amino acids and has a calculated molecular mass of 84.8 kDa. The recombinant protein migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nek3 NIMA (never in mitosis gene a)-related expressed kinase 3 [ Mus musculus ]
• Official Symbol: NEK3
• Synonyms: NEK3; NIMA (never in mitosis gene a)-related expressed kinase 3; serine/threonine-protein kinase Nek3; nimA-related protein kinase 3; NIMA-related expressed kinase 3; never in mitosis A-related kinase 3
• Gene ID: 23954
• mRNA Refseq: NM_001162947
• Protein Refseq: NP_001156419