Recombinant Mouse Ngef Protein, Myc/DDK-tagged

• Cat.No.: Ngef-4405M
• Product Overview: Purified recombinant protein of mouse full-length neuronal guanine nucleotide exchange factor (Ngef), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 82.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ngef neuronal guanine nucleotide exchange factor [ Mus musculus (house mouse) ]
• Official Symbol: Ngef
• Synonyms: Ngef; neuronal guanine nucleotide exchange factor; Tim; ephe; Tims2; ephexin; ephexin-1; eph-interacting exchange protein; transforming immortalised mammary epithelial sequence 2
• Gene ID: 53972
• mRNA Refseq: NM_001111314
• Protein Refseq: NP_001104784
• UniProt ID: Q8CHT1