Recombinant Mouse Nsun6 Protein, Myc/DDK-tagged

• Cat.No.: Nsun6-4512M
• Product Overview: Purified recombinant protein of mouse full-length NOL1/NOP2/Sun domain family member 6 (Nsun6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 52.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nsun6 NOL1/NOP2/Sun domain family member 6 [ Mus musculus (house mouse) ]
• Official Symbol: Nsun6
• Synonyms: NSUN6; NOL1/NOP2/Sun domain family member 6; putative methyltransferase NSUN6; NOL1/NOP2/Sun domain family 6; NOPD1; 4933403D21Rik; 4933414E04Rik
• Gene ID: 74455
• mRNA Refseq: NM_001165941
• Protein Refseq: NP_001159413
• UniProt ID: Q7TS68