Recombinant Mouse Nt5e protein(Met1-Lys549), His-tagged

• Cat.No.: Nt5e-2302M
• Product Overview: Recombinant Mouse NT5E (NP_035981.1) without the propeptide, corresponding to the amino acid (Met 1-Lys 549) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Lys549
• Form: Lyophilized from 20 mM Tris, 500 mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >10, 000 pmol/min/μg.
• Molecular Mass: The secreted recombinant mouse NT5E consists of 532 amino acids and has a calculated molecular mass of 59.4 kDa which is also detected in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nt5e 5 nucleotidase, ecto [ Mus musculus ]
• Official Symbol: Nt5e
• Synonyms: NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; NT; Nt5; eNT; CD73; AI447961; 2210401F01Rik
• Gene ID: 23959
• mRNA Refseq: NM_011851
• Protein Refseq: NP_035981

Reviews

11/20/2022

the manufacturer's support plays a crucial role in the successful implementation of trials involving the NT5E protein.

09/01/2017

The manufacturer maintains stringent quality control measures to ensure the purity and stability of the protein, minimizing experimental variability and maximizing the accuracy of the data obtained.

01/25/2017

Their responsiveness to inquiries and willingness to address concerns contribute to a smooth and productive research experience.

Q&As

Can NT5E be used as a biomarker for certain diseases?

Yes, elevated levels of NT5E have been observed in various diseases, making it a potential biomarker for conditions such as cancer and autoimmune disorders.

How does NT5E contribute to inflammation?

NT5E-generated adenosine has anti-inflammatory effects, and modulating NT5E activity is being explored as a potential therapeutic strategy for inflammatory conditions.

Is NT5E associated with autoimmune diseases?

Yes, NT5E is implicated in autoimmune diseases, and targeting its activity is being investigated for potential therapeutic interventions.

Are there any FDA-approved drugs targeting NT5E for cancer treatment?

As of my last knowledge update in January 2022, there were no FDA-approved drugs specifically targeting NT5E, but research in this area is ongoing.

How is NT5E linked to neurological disorders?

NT5E-generated adenosine is neuroprotective, and research is exploring its potential in the treatment of neurodegenerative disorders.