Recombinant Mouse Nthl1 Protein, Myc/DDK-tagged

• Cat.No.: Nthl1-4519M
• Product Overview: Purified recombinant protein of mouse full-length nth (endonuclease III)-like 1 (E.coli) (Nthl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 33.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nthl1 nth (endonuclease III)-like 1 (E.coli) [ Mus musculus (house mouse) ]
• Official Symbol: Nthl1
• Synonyms: NTHL1; nth (endonuclease III)-like 1 (E.coli); endonuclease III-like protein 1; thymine glycol DNA glycosylase/AP lyase; Nth1; Octs3
• Gene ID: 18207
• mRNA Refseq: NM_008743
• Protein Refseq: NP_032769
• UniProt ID: O35980