Recombinant Mouse Ntrk3 protein(Met1-Thr429), His-tagged

• Cat.No.: Ntrk3-625M
• Product Overview: Recombinant Mouse TrkC (NP_032772.3) (Met 1-Thr 429) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Thr429
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse NTRK3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NT3, The EC50 of biotinylated human NT3 is 0.04-0.08 μg/ml.
• Molecular Mass: The secreted recombinant mouse TrkC comprises 409 amino acids and has a calculated molecular mass of 46.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ntrk3 neurotrophic tyrosine kinase, receptor, type 3 [ Mus musculus ]
• Official Symbol: Ntrk3
• Synonyms: NTRK3; neurotrophic tyrosine kinase, receptor, type 3; NT-3 growth factor receptor; trk-C; GP145-TrkC; neurotrophin-3; trkC tyrosine kinase; TrkC; AW125844; Ntrk3_tv3
• Gene ID: 18213
• mRNA Refseq: NM_008746
• Protein Refseq: NP_032772