Recombinant Mouse Pou3f2 Protein, Myc/DDK-tagged

• Cat.No.: Pou3f2-5020M
• Product Overview: Purified recombinant protein of mouse full-length POU domain, class 3, transcription factor 2 (Pou3f2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcription factor that plays a key role in neuronal differentiation Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides. Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 47.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pou3f2 POU domain, class 3, transcription factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Pou3f2
• Synonyms: POU3F2; POU domain, class 3, transcription factor 2; octamer-binding protein 7; brain-2 class III POU-domain protein; octamer-binding transcription factor 7; brain-specific homeobox/POU domain protein 2; nervous system-specific octamer-binding transcription factor N-Oct-3; Brn2; Otf7; Brn-2; OTF-7; oct-7; 9430075J19Rik; A230098E07Rik
• Gene ID: 18992
• mRNA Refseq: NM_008899
• Protein Refseq: NP_032925
• UniProt ID: P31360