Recombinant Mouse Rprd1b Protein, Myc/DDK-tagged

• Cat.No.: Rprd1b-5600M
• Product Overview: Purified recombinant protein of mouse full-length regulation of nuclear pre-mRNA domain containing 1B (Rprd1b), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 36.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rprd1b regulation of nuclear pre-mRNA domain containing 1B [ Mus musculus (house mouse) ]
• Official Symbol: Rprd1b
• Synonyms: RPRD1B; regulation of nuclear pre-mRNA domain containing 1B; regulation of nuclear pre-mRNA domain-containing protein 1B; cell cycle-related and expression-elevated protein in tumor; cell-cycle related and expression-elevated protein in tumor; Crept; RP23-396G1.1; 2610304G08Rik; 2810446G03Rik
• Gene ID: 70470
• mRNA Refseq: NM_027434
• Protein Refseq: NP_081710
• UniProt ID: Q9CSU0