Recombinant Mouse Tnfrsf1a protein(Met1-Ala212), His-tagged

• Cat.No.: Tnfrsf1a-3306M
• Product Overview: Recombinant Mouse TNFRSF1A (NP_ 035739.2) (Met 1-Ala 212) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ala212
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.28 μg/mL. 3. Immobilized mouse TNFa at 10 μg/mL (100 μl/well) can bind biotinylated mouse TNFRSF1A-his. The EC50 of biotinylated mouse TNFRSF1A-his is 0.13 μg/mL.
• Molecular Mass: The secreted recombinant mouse TNFRSF1A consists of 194 amino acids and has a predicted molecular mass of 21.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A is approximately 35 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnfrsf1a tumor necrosis factor receptor superfamily, member 1a [ Mus musculus ]
• Official Symbol: Tnfrsf1a
• Synonyms: TNFRSF1A; tumor necrosis factor receptor superfamily, member 1a; tumor necrosis factor receptor superfamily member 1A; p60; TNF-RI; TNFR-I; TNF-alpha-R1; TNF receptor alpha chain; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I; FPF; p55; TNF-R; TNFAR; TNFRI; Tnfr1; p55-R; CD120a; TNF-R1; TNFR60; Tnfr-2; TNF-R-I; TNF-R55; TNFRp55; TNF-alphaR1; TNFalpha-R1
• Gene ID: 21937
• mRNA Refseq: NM_011609
• Protein Refseq: NP_035739