Recombinant Mouse Ube2c Protein, Myc/DDK-tagged

• Cat.No.: Ube2c-6781M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2C (Ube2c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 19.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2c ubiquitin-conjugating enzyme E2C [ Mus musculus (house mouse) ]
• Official Symbol: Ube2c
• Synonyms: UBE2C; ubiquitin-conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; ubcH10; ubiquitin-protein ligase C; ubiquitin carrier protein C; D2Ertd695e; 1110015A16Rik
• Gene ID: 68612
• mRNA Refseq: NM_026785
• Protein Refseq: NP_081061
• UniProt ID: Q9D1C1