Recombinant Mouse Wee1 protein(1-646aa), His-tagged

• Cat.No.: Wee1-6652M
• Product Overview: Recombinant Mouse Wee1 protein(P47810)(1-646aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Mouse
• Tag: N-His
• Protein length: 1-646aa
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 77.5 kDa
• AASequence: MSFLSRQQPPPTRRVGAAYSLRQKL IFSPGSDCEEEEEEEEEGSGHSTGE DSAFQEPDSPLPSARSPAEAEAERR RRSPGAEPSSPGELEDDLLLQGGGG GAQAAGGGAEGDSWEEEGFGSSSPV KSPSTAYFLSSPFSPVRCGGPGDAS PQGCGAPRAMDDPCSPQPDYPSTPP HKTFRKLRLFDTPHTPKSLLSKARV IDSGSVKLRGSSLFMDTEKSGKREF DTRQTPQVNINPFTPDPVLLHSSGR CRGRKRAYFNDSSEDMEASDYEFED ETRPAKRITITESNMKSRYTTEFHE LEKIGSGEFGSVFKCVKRLDGCIYA IKRSKKPLAGSVDEQNALREVYAHA VLGQHPHVVRYFSAWAEDDHMLIQN EYCNGGSLADAISENYRVMSYLTEV ELKDLLLQVGRGLRYIHSMSLVHMD IKPSNIFISRTSIPNAVSEEGDEDD WISNKVMFKIGDLGHVTRISSPQVE EGDSRFLANEVLQENYSHLPKADIF ALALTVVCAAGAEPLPRNGEQWHEI RQGRLPRIPQVLSQEVTELLRVMIH PDPERRPSAMELVKHSVLLSASRKS AEQLRIELNAEKFKNSLLQKELKKA QMAAKVAAEERALFTDRMATRSTTQ SNRTSRLIGKKMNRSVSLTIY
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Gene Information

• Gene Name: Wee1 WEE 1 homolog 1 (S. pombe) [ Mus musculus ]
• Official Symbol: Wee1
• Synonyms: WEE1; WEE 1 homolog 1 (S. pombe); wee1-like protein kinase; wee1A kinase; WEE1 tyrosine kinase; Wee1A
• Gene ID: 22390
• mRNA Refseq: NM_009516
• Protein Refseq: NP_033542

Reviews

07/10/2019

Dependable results. Boosts our research credibility.

12/12/2018

Expertise shines. Essential for our research endeavors.

02/12/2018

Reliable partner in experiments. Highly recommended for research.

Q&As

How does WEE1 contribute to DNA damage response?

It plays a role in DNA damage response by delaying cell cycle progression to allow DNA repair.

What is the impact of altered WEE1 activity on cancer development?

Altered WEE1 activity is associated with the progression of various cancers.

What potential therapeutic applications arise from targeting WEE1 in cancer treatment?

Targeting WEE1 in cancer therapy could exploit its role in cell cycle regulation to inhibit tumor growth.

How do genetic variations in WEE1 affect cell division?

Genetic mutations in WEE1 can lead to uncontrolled cell division and genomic instability.

What role does WEE1 play in checkpoint regulation?

WEE1 is critical in checkpoint regulation, ensuring cells do not divide with damaged DNA.

How does WEE1 interact with other cell cycle proteins?

WEE1 works in conjunction with other cell cycle proteins to regulate the timing of cell division.

What is the primary role of WEE1 in cell cycle control?

WEE1 is a kinase that inhibits cell cycle progression, particularly at the G2/M checkpoint.