Recombinant Mouse Wtap Protein, Myc/DDK-tagged

• Cat.No.: Wtap-7012M
• Product Overview: Purified recombinant protein of Mouse Wilms tumour 1-associating protein (Wtap), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Acts as a key regulator of m6A methylation by promoting m6A methylation of mRNAs at the 3'-UTR. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 17.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wtap Wilms tumour 1-associating protein [ Mus musculus (house mouse) ]
• Official Symbol: Wtap
• Synonyms: WTAP; Wilms tumour 1-associating protein; pre-mRNA-splicing regulator WTAP; WT1-associated protein; female-lethal(2)D homolog; wilms tumor 1-associating protein; 2810408K05Rik; 9430038B09Rik
• Gene ID: 60532
• mRNA Refseq: NM_175394
• Protein Refseq: NP_780603
• UniProt ID: Q9ER69