Recombinant Rat ABCB4 Protein Pre-coupled Magnetic Beads

• Cat.No.: ABCB4-52R-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Rat
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Abcb4 ATP-binding cassette, subfamily B (MDR/TAP), member 4 [ Rattus norvegicus ]
• Official Symbol: ABCB4
• Gene ID: 24891
• mRNA Refseq: NM_012690.2
• Protein Refseq: NP_036822.1
• UniProt ID: Q08201

Reviews

12/07/2022

I purchased this protein product for Western blotting, and the feedback results are not bad, and it can meet my experimental needs.

06/11/2019

I have received my customized recombinant ABCB4 protein, and the delivery cycle is short

Q&As

Do you offer low endotoxin recombinant ABCB4 protein?

The endotoxin level of recombinant protein can be customized according to customer needs, and you can communicate with our technical experts in detail.

Is the tag of the recombinant ABCB4 protein at the N-terminus or the C-terminus?

We provide these two types of recombinant proteins, you can buy or customize according to your needs.

The purity of ABCB4-1091M is >80%, can I order ABCB4 protein with higher purity?

We provide recombinant protein products with different purities, and you can communicate with our technical experts about your customized needs.

How to choose the two product forms of ABCB4-397R?

Both have their own advantages, and the freeze-dried form is more stable than the liquid form, but the freeze-drying process may cause certain damage to the structure and activity of the protein.

ABCB4 protein is expressed in hepatocytes, is it related to the formation of bile?

Bile contains three main lipids: cholesterol, phospholipids and bile acids. ABCB4 protein participates in phospholipid transport and is crucial for the formation of bile.

Is the ABCB4 protein the same as MDR3?

ABCB4 protein is also known as multidrug resistance protein 3 (MDR3).

How long should I incubate with the primary antibody at room temperature when performing a western blot for ABCB4?

It is generally recommended to incubate at room temperature for about two hours, but the exact time may also vary.

How should I quantify the results when I perform a western blot of ABCB4 protein?

Quantification can be performed in the linear range of detection using ImageJ software.

The ABCB4 protein (Cat.No. : ABCB4-397R) has two product forms, can I specify the lyophilized powder product to be shipped?

If you have requirements for the product format, please note when placing an order.

I want to use ABCB4 protein for Western blotting, how should I determine the concentration of proteins in the experiment?

You can use the Uptima bicinchoninic acid protein assay to determine the protein concentrations.