Recombinant Rat Agrin

• Cat.No.: Agrn-1789R

Specification

• Cat. No.: Agrn-1789R
• Description: Agrin is a large proteoglycan whose best characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the AGRIN gene. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is major proteoglycan component in glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions.
• Molecular Weight: The predicted molecular weight of Recombinant Rat Agrin (C-Terminal) is Mr 90 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 100 kDa.
• Source: Sf 21 Insect Cells.
• State Of Matter: Lyophilized.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°Cto -70°C. After aseptic reconstitution, this protein may be stored at 2°Cto 8°Cfor one month or at -20°Cto -70°Cin a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: Agrn agrin [ Rattus norvegicus ]
• Synonyms: Agrn; agrin;AGR
• Gene ID: 25592
• mRNA Refseq: NM_175754
• Protein Refseq: NP_786930
• UniProt ID: P25304
• Chromosome Location: 5q36
• Pathway: ECM-receptor interaction
• Function: acetylcholine receptor regulator activity; laminin binding

Reviews

11/23/2022

The packaging was secure, and the proteins maintained their integrity and activity upon arrival.

12/03/2021

I highly recommend their products for researchers seeking reliable reagents.

09/14/2021

They offer excellent value for the price.

07/09/2021

We rely on this protein for its consistent performance.

05/22/2021

I am extremely satisfied with the prompt delivery of the proteins.

Q&As

Can AGRN be used as a diagnostic marker for neurological diseases?

The measurement of AGRN levels or its isoforms in biological fluids or tissues may hold potential as diagnostic markers for certain neurological disorders, although further research is needed.

How is AGRN involved in neuromuscular junction formation?

AGRN interacts with specific receptors on the muscle and nerve cells, initiating a signaling cascade that leads to the clustering of acetylcholine receptors and the formation and maintenance of the NMJ.

Is AGRN involved in other organ systems besides the neuromuscular junction?

Yes, AGRN has been shown to have roles outside of the NMJ, including in the development of the central nervous system, kidneys, and cardiovascular system.

What are the isoforms of AGRN?

AGRN exists in multiple isoforms generated by alternative splicing. The most well-known isoforms include the neural isoforms (Agrin-N) and the muscle-specific isoforms (Agrin-M).

What is the structure of AGRN?

AGRN consists of several functional domains, including N-terminal laminin G-like (LG) domains, central EGF-like repeats, a C-terminal domain, and a heparin-binding site.

How is AGRN involved in cancer progression?

AGRN has been found to promote tumor cell migration, invasion, and metastasis by interacting with receptors and signaling pathways that regulate cell adhesion, migration, and angiogenesis.

How is AGRN expression regulated?

AGRN expression is tightly regulated during development and can be influenced by various signaling pathways, including Wnt, BMP, and Notch signaling.

What are the receptor molecules for AGRN?

AGRN interacts with two major receptors: muscle-specific kinase (MuSK) on the postsynaptic membrane and low-density lipoprotein receptor-related protein 4 (LRP4) on the presynaptic membrane.

How is AGRN involved in synaptic plasticity?

AGRN plays a role in regulating synaptic plasticity by modulating the structure and function of synapses, including their strength and stability.

How is AGRN involved in synaptic maturation?

AGRN regulates the maturation and stabilization of synapses by promoting the clustering of postsynaptic receptors and facilitating the development of pre- and postsynaptic specializations.