Recombinant Rat Agtr1a protein, His & GST-tagged

• Cat.No.: Agtr1a-1768R
• Product Overview: Recombinant Rat Agtr1a aa. (Asp263~Glu359 (Accession # P25095)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Receptor for and mediator of vascular remodeling effects of angiotensin II.
• Source: E. coli
• Species: Rat
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40.9kDa
• Protein length: Asp263~Glu359 (Accession # P25095)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 9.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Agtr1a angiotensin II receptor, type 1a [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Agtr1a
• Synonyms: Agtr1a; angiotensin II receptor, type 1a; AT1; AT1A; AT1R; Agtr1; type-1A angiotensin II receptor; angiotensin II type-1A receptor; angiotensin receptor 1a; vascular type-1 angiotensin II receptor
• Gene ID: 24180
• mRNA Refseq: NM_030985.4
• Protein Refseq: NP_112247.2
• UniProt ID: P25095

Reviews

07/22/2022

The customer service was fantastic—helpful, friendly, and made the ordering process a breeze!

09/18/2021

Quick delivery and secure packaging ensured my recombinant proteins arrived in perfect condition.

04/10/2021

Super impressed with the reliable recombinant proteins I received at such a reasonable cost

02/24/2021

The stability of this protein is unparalleled. It remains intact and active even under challenging conditions, ensuring reliable and reproducible results.

08/20/2020

Couldn't be happier with the high-quality recombinant proteins I got at such an amazing price.

Q&As

How does AGTR1A influence vascular tone?

AGTR1A activation leads to vasoconstriction by stimulating smooth muscle cell contraction, thereby affecting vascular tone and blood pressure.

How can protein-protein interactions involving AGTR1A be studied in the lab?

Techniques such as co-immunoprecipitation (Co-IP) or proximity-based assays like the fluorescence resonance energy transfer (FRET) can be utilized to investigate protein-protein interactions involving AGTR1A.

Are there specific ligands or agonists for AGTR1A?

Angiotensin II is the primary ligand for AGTR1A, but there are also selective agonists and antagonists that can modulate its activity for research and therapeutic purposes.

Can AGTR1A polymorphisms influence drug response?

Genetic polymorphisms in AGTR1A have been associated with variable responses to ARBs and other antihypertensive medications, affecting treatment outcomes.

How can AGTR1A signaling pathways be investigated in the lab?

Lab researchers can use techniques like phospho-specific antibodies, ELISA assays, or luciferase reporter assays to assess the activation and downstream signaling of AGTR1A pathways.

Can AGTR1A contribute to insulin resistance and metabolic disorders?

AGTR1A signaling has been implicated in insulin resistance, glucose metabolism, and the development of metabolic disorders such as obesity and diabetes.

What signaling pathways are activated by AGTR1A?

AGTR1A activates various signaling pathways, including phospholipase C (PLC), protein kinase C (PKC), mitogen-activated protein kinases (MAPKs), and intracellular calcium release.

What are the structural features of AGTR1A that allow ligand binding?

AGTR1A has extracellular domains and specific amino acid residues within the transmembrane region that facilitate angiotensin II binding and receptor activation.

How is AGTR1A activated?

AGTR1A is activated when angiotensin II binds to the receptor, leading to conformational changes that initiate intracellular signaling cascades.

What is the process for screening compounds targeting AGTR1A in the lab?

High-throughput screening (HTS) methods, such as cell-based assays or binding assays using radiolabeled ligands, can be employed to identify and evaluate potential compounds targeting AGTR1A.