Recombinant Rat CNTF protein(Met1-Met200)

• Cat.No.: Cntf-579R
• Product Overview: Recombinant Rat CNTF (P20294) (Met 1-Met 200) was expressed and purified in E. coli.

Specification

• Source: E. coli
• Species: Rat
• Tag: N/A
• Protein length: Met1-Met200
• Form: Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized rat CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) with a linear range of 1.56-50.0 ng/ml. 2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 10-100 ng/ml.
• Molecular Mass: The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cntf ciliary neurotrophic factor [ Rattus norvegicus ]
• Official Symbol: CNTF
• Synonyms: CNTF; ciliary neurotrophic factor; ciliary neurotropic factor
• Gene ID: 25707
• mRNA Refseq: NM_013166
• Protein Refseq: NP_037298