Recombinant Rat Egf Protein, His-tagged

• Cat.No.: Egf-1410R
• Product Overview: Recombinant Rat Egf Protein (Asn974-Arg1026) with N-His tag was expressed in E. coli.

Specification

• Description: Enables epidermal growth factor receptor binding activity. Involved in several processes, including positive regulation of DNA biosynthetic process; positive regulation of ERK1 and ERK2 cascade; and positive regulation of fibroblast proliferation. Acts upstream of or within regulation of protein transport. Located in extracellular space. Used to study perinatal necrotizing enterocolitis. Biomarker of acute kidney failure. Human ortholog(s) of this gene implicated in several diseases, including Zollinger-Ellison syndrome; hepatobiliary system cancer (multiple); high grade glioma (multiple); lung non-small cell carcinoma (multiple); and primary hypomagnesemia (multiple). Orthologous to human EGF (epidermal growth factor).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 36.1 kDa
  Accurate Molecular Mass: 38 kDa
• Protein length: Asn974-Arg1026
• Purity: > 95%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Egf epidermal growth factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Egf
• Synonyms: EGF; epidermal growth factor; pro-epidermal growth factor
• Gene ID: 25313
• mRNA Refseq: NM_012842
• Protein Refseq: NP_036974
• UniProt ID: P07522