Recombinant Rat Itln1 protein, His-tagged

• Cat.No.: Itln1-5638R
• Product Overview: Recombinant Rat Itln1 aa. (Ser101~Gly292 (Accession # Q499T8)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 22 kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser101~Gly292 (Accession # Q499T8)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.1.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Itln1 intelectin 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Itln1
• Synonyms: Itln1; intelectin 1; intelectin-1; intelectin 1 (galactofuranose binding); intelectin 1 (galactofuranose binding)-like
• Gene ID: 498284
• mRNA Refseq: NM_001034946.1
• Protein Refseq: NP_001030118.1
• UniProt ID: Q499T8

Reviews

12/29/2021

ITLN1 activity is good, and the required biological activity experiments can be carried out effectively.

12/13/2021

The results show that the protein has good crystallinity, which is helpful for crystallographic study.

07/20/2021

Its high solubility allows for easy preparation of the required concentration.

Q&As

What are the directions worth paying attention to for ITLN1 in future research?

In future studies, it is worth paying attention to the relationship between structure and function, expression regulation, association with other proteins or diseases, and therapeutic potential. At the same time, further research is needed on the similarities and differences between them and other protein inhibitors, as well as their differences in different species.

How about the ITLN1 expression regulated?

The expression level of ITLN1 is regulated by a variety of factors, including processes such as gene transcription, translation, and post-translational modifications, as well as hormones, nutrients, and other environmental factors in the body.

Does ITLN1 have other biological activities?

Yes, ITLN1 may have other biological activities in addition to trypsin inhibitory activity. For example, it may play a role in processes such as immune regulation, cell growth, and apoptosis.

Are there differences in ITLN1 among different species?

Yes, there may be some differences in ITLN1 in different species, such as different degrees of variation in structure, function, and expression levels.

How does the synthesis and secretion of ITLN1 in the human body take place?

The synthesis and secretion of ITLN1 in the human body involves multiple steps and complex regulatory mechanisms, including gene transcription, translation, post-translational modification and secretion.

What are the similarities and differences between ITLN1 and other protein inhibitors?

There are certain similarities and differences between ITLN1 and other protein inhibitors in terms of structure, function, and mechanism of action. For example, other protein inhibitors may have different mechanisms of inhibition and ranges of action.