Recombinant Rat Lif Protein, His-tagged

• Cat.No.: Lif-1739R
• Product Overview: Recombinant Rat Lif Protein (Leu25-Phe202) with N-His tag was expressed in E. coli.

Specification

• Description: Enables leukemia inhibitory factor receptor binding activity. Involved in several processes, including negative regulation of angiogenesis; positive regulation of corticotropin secretion; and transdifferentiation. Located in extracellular space. Biomarker of brain ischemia and congestive heart failure. Orthologous to human LIF (LIF interleukin 6 family cytokine).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.3 kDa
  Accurate Molecular Mass: 25 kDa
• Protein length: Leu25-Phe202
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Lif leukemia inhibitory factor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Lif
• Synonyms: LIF; leukemia inhibitory factor; cholinergic neuronal differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor)
• Gene ID: 60584
• mRNA Refseq: NM_022196
• Protein Refseq: NP_071532
• UniProt ID: P17777