Recombinant Rat Pdk1 Protein, His-tagged

• Cat.No.: Pdk1-1932R
• Product Overview: Recombinant Rat Pdk1 Protein (Val172-Pro399) with N-His tag was expressed in E. coli.

Specification

• Description: Enables ATP binding activity; identical protein binding activity; and protein kinase activity. Involved in cell surface receptor signaling pathway and peptidyl-serine phosphorylation. Part of pyruvate dehydrogenase complex. Orthologous to human PDK1 (pyruvate dehydrogenase kinase 1).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.6 kDa
  Accurate Molecular Mass: 30 kDa
• Protein length: Val172-Pro399
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Pdk1 pyruvate dehydrogenase kinase, isozyme 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Pdk1
• Synonyms: PDK1; pyruvate dehydrogenase kinase, isozyme 1; PDK p48; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase, isoenzyme 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; MGC108625
• Gene ID: 116551
• mRNA Refseq: NM_053826
• Protein Refseq: NP_446278
• UniProt ID: Q5FVT5