Recombinant Rat PRF1 Protein (25-554 aa), His-tagged

• Cat.No.: PRF1-738R
• Product Overview: Recombinant Rat PRF1 Protein (25-554 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.

Specification

• Description: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Can insert into the mbrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
• Source: E. coli
• Species: Rat
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 62.9 kDa
• Protein length: 25-554 aa
• AA Sequence: ATRSECKQNHKFVPGVWAAGEGVDV TTLRRSSSFPVNTGKFLRPDRTCTL CKNALMNDGIQRLPVAIAHWRPHGS HCQRNVATTKVSSTEGVAREAAANI NNDWRAGLDVNPKPEANVHVSVAGS HSKIANFAAEKAHQDQYNFNTDTVE CRMYSFRLAQKPPLHPDFRKALKNL PHNFNSSTEHAYRRLISSYGTHFIT AVDLGGRVSVLTALRTCQLTLDGLT ADEVGDCLSVEAQVSIGAQASVSSE YKACEEKKKQHKIATSFHQTYRERH VEVLGGPLDSSNDLLFGNQATPEHF STWIASLPTRPDVVDYSLEPLHILL EDSDPKREALRQAISHYVMSRARWR DCNRPCRAGQHKSSRDSCQCVCQDS NVTNQDCCPRQRGLAKLMVRNFQAK GLWGDYITSTDAYLKVFFGGQEIRT GVVWNNNHPSWSDKMDFGNVLLSTG GPLRVQVWDADNGWDDDLLGTCDKS PKSGFHEVNCPLNHGSIKFIYQANC LPDLTGETCLEYAPQGLLGDPRGNR SGAVW
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Prf1 perforin 1 (pore forming protein) [ Rattus norvegicus ]
• Official Symbol: PRF1
• Synonyms: PRF1; perforin-1; P1; cytolysin; Cyta; RATCYTA; MGC108712
• Gene ID: 50669
• mRNA Refseq: NM_017330
• Protein Refseq: NP_059026
• UniProt ID: P35763