Recombinant Rat Tek protein(Met4-Leu743), hFc-tagged

• Cat.No.: Tek-1360R
• Product Overview: Recombinant Rat TEK (NP_001099207.1)(Met 4-Leu 743) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met4-Leu743
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized S1h-3C-mANGPT2 at 10 μg/mL (100 μL/well) can bind ratTEK-Fc. The EC50 of ratTEK-Fc is 0.26-0.62μg/mL.
• Molecular Mass: The recombinant rat TEK/Fc is a disulfide-linked homodimer. The reduced monomer comprises 962 amino acids and predicts a molecular mass of 108 kDa. The apparent molecular mass of the rat TEK/Fc monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tek TEK tyrosine kinase, endothelial [ Rattus norvegicus ]
• Official Symbol: Tek
• Synonyms: TEK; TEK tyrosine kinase, endothelial; angiopoietin-1 receptor; endothelial-specific receptor tyrosine kinase; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; Tie2; Tie-2
• Gene ID: 89804
• mRNA Refseq: NM_001105737
• Protein Refseq: NP_001099207

Reviews

06/07/2020

Outstanding service! Staff expertise helped us choose the perfect protein.

10/31/2019

Seamless delivery process—ordered, received, and ready for experiments.

03/14/2018

Courteous and efficient customer service made our purchase hassle-free.

Q&As

How does the TEK gene influence vascular development during embryogenesis, and what are the key molecular players involved?

The TEK gene is crucial for vascular development during embryogenesis, coordinating with other genes and signaling pathways to ensure proper formation of the vascular system.

What role does the TEK gene play in pathological angiogenesis, particularly in diseases like cancer?

The TEK gene is implicated in pathological angiogenesis in diseases like cancer, where dysregulation of TIE2 signaling can contribute to aberrant blood vessel formation.

How does the TEK gene contribute to the modulation of vascular permeability?

The TEK gene, through TIE2 signaling, regulates vascular permeability by influencing the integrity of endothelial cell junctions and controlling the passage of molecules across blood vessels.

How does the TEK gene contribute to the maintenance of vascular quiescence, and what molecular processes are involved?

The TEK gene contributes to the maintenance of vascular quiescence by suppressing endothelial cell activation and inflammation, preserving the stability of blood vessels.

How does the TEK gene participate in the regulation of immune responses in the context of vascular inflammation?

The TEK gene, through TIE2 signaling, influences immune responses by modulating interactions between endothelial cells and immune cells during vascular inflammation.