Recombinant Rhesus Macaque APOBEC3B Protein Pre-coupled Magnetic Beads

• Cat.No.: APOBEC3B-190R-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Rhesus Macaque
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: APOBEC3B apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: APOBEC3B
• Synonyms: APOBEC3B; probable DNA dC->dU-editing enzyme APOBEC-3B; apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3B
• Gene ID: 100628312
• mRNA Refseq: NM_001246230
• Protein Refseq: NP_001233159
• UniProt ID: F6M3K5

Reviews

09/23/2022

The precise and reproducible results obtained using the APOBEC3B protein have significantly enhanced the accuracy and validity of my research findings.

12/20/2019

Its exceptional performance, in combination with its clear and distinct protein bands, make it an absolute must-have for researchers looking to achieve accurate and reliable results in their experiments.

03/09/2019

The superior sensitivity and specificity exhibited by the APOBEC3B protein have greatly simplified my research and enabled precise protein characterization.

10/15/2017

The bands of proteins detected were remarkably clear, allowing for easy interpretation and analysis of protein expression or modifications.

Q&As

Is APOBEC3B involved in any diseases or conditions?

Aberrant expression or dysregulation of APOBEC3B has been implicated in various types of cancer, including breast, bladder, and lung cancer. Its overexpression can contribute to genetic mutations and genomic instability in cancer cells.

Can APOBEC3B gene mutations cause genetic disorders?

In general, APOBEC3B mutations are not associated with genetic disorders. However, mutations in other members of the APOBEC3 family, such as APOBEC3A, have been linked to rare genetic disorders like hypermutation syndrome.

What techniques are used to study APOBEC3B?

Research on APOBEC3B involves various techniques such as gene expression analysis, protein assays, biochemical assays, next-generation sequencing, CRISPR/Cas9-mediated gene editing, immunohistochemistry, and animal models. These techniques help researchers investigate its expression, activity, protein-protein interactions, and functional consequences.

Are there any ongoing clinical trials investigating APOBEC3B-related therapies?

Currently, there are no registered clinical trials specifically targeting APOBEC3B in cancer. However, considering the growing interest in understanding its role in cancer development, it is possible that clinical trials will be initiated in the future.

Is APOBEC3B specific to antiviral defense, or does it have other cellular functions?

APOBEC3B primarily functions in antiviral defense by inducing hypermutation in viral genomes. However, recent studies suggest it may also play a role in DNA repair processes and contribute to genome instability in cancer.

Does APOBEC3B have any known interactions with viruses other than retroviruses?

While APOBEC3B's primary role is to restrict retroviruses, studies have shown its activity against other viruses as well. These include hepatitis B virus (HBV), human papillomavirus (HPV), hepatitis C virus (HCV), and others. However, the extent of its antiviral activity against non-retroviral viruses is still being explored.

Is APOBEC3B expression linked to drug resistance?

There is evidence suggesting that APOBEC3B expression is associated with increased drug resistance in certain cancers, such as breast and lung cancer. Its mutagenic activity can contribute to the acquisition of drug-resistant mutations, posing a challenge for effective cancer treatment.

Can APOBEC3B be used as a diagnostic or prognostic marker for cancer?

APOBEC3B expression levels have been evaluated as potential diagnostic or prognostic markers in various cancers. However, more research is needed to establish its clinical utility.

How is APOBEC3B regulated in the cell?

APOBEC3B expression can be regulated by various factors, including different signaling pathways and epigenetic modifications. The precise mechanisms regulating its expression are still under investigation.

Are there any known therapeutic approaches targeting APOBEC3B in cancer treatment?

While there are currently no approved therapies specifically targeting APOBEC3B in cancer, understanding its role in tumor development and progression has opened up new avenues for potential therapeutic interventions. For example, targeting DNA repair pathways affected by APOBEC3B activity or combining APOBEC3B inhibition with other treatments may hold promise for future clinical applications.

Is APOBEC3B expressed in normal tissues as well?

Yes, APOBEC3B is expressed at low levels in various normal tissues, such as breast, lung, and reproductive organs. However, its expression is significantly upregulated in cancer tissues, contributing to the malignant phenotype.

Does APOBEC3B have any known roles outside of antiviral defense and cancer?

While APOBEC3B's primary functions are related to antiviral defense and cancer, emerging research suggests its involvement in other biological processes. For example, it may play a role in DNA damage response, immune regulation, and autoimmune diseases. However, further investigation is required to fully understand its broader functions.

What are the mechanisms regulating the expression of APOBEC3B in cancer cells?

The regulation of APOBEC3B expression in cancer cells is complex and not fully understood. However, various transcription factors, DNA methylation patterns, histone modifications, and signaling pathways have been implicated in its dysregulation in cancer cells.

Are there any known genetic variants of the APOBEC3B gene?

Yes, genetic variants and polymorphisms of the APOBEC3B gene have been identified. Some of these variants have been associated with altered protein expression and activity.

Are there any therapeutic strategies targeting APOBEC3B for cancer treatment?

Targeting APOBEC3B directly for cancer treatment has proven challenging due to its essential role in antiviral defense. However, researchers are exploring strategies to inhibit its activity selectively in cancer cells to reduce mutagenesis without compromising the immune system.

Are there any known inhibitors or activators of APOBEC3B?

Currently, there are no specific inhibitors or activators that target APOBEC3B directly. However, certain molecules or compounds that modulate other proteins in the same pathway or interactome as APOBEC3B may indirectly affect its activity.

Can environmental factors influence APOBEC3B expression or activity?

Yes, certain environmental factors like exposure to certain chemicals or chronic viral infections can upregulate APOBEC3B expression. This increased activity may contribute to higher mutation rates and cancer development.

Does APOBEC3B interact with other proteins or pathways in the cell?

APOBEC3B has been found to interact with several cellular proteins and pathways involved in DNA repair and replication. These interactions may influence its function and contribute to genome maintenance and stability.