Recombinant Rhesus macaque CD4 protein, Fc-tagged

• Cat.No.: CD4-740R
• Product Overview: Recombinant Rhesus macaque CD4 (Lys26-Trp390) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
• Source: HEK293
• Species: Rhesus macaque
• Tag: human IgG1 Fc
• Predicted N Terminal: Lys26
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 67.0 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Lys26-Trp390
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD4
• Official Symbol: CD4
• Synonyms: CD4mut
• Gene ID: 713807
• mRNA Refseq: NM_001042662.1
• Protein Refseq: NP_001036127.1
• UniProt ID: P16003

Reviews

01/08/2031

The CD4 proteins purchased from your company is suitable for ELISA.

02/06/2023

Protein products are put into experiments with good effect and reliable experimental results.

01/22/2022

The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

Q&As

What is the connection between CD4 and glycoprotein GP120?

CD4 binds to glycoprotein GP120.

Where is CD4 primarily expressed?

CD4 is mainly expressed in T cells and thymocytes.

Where is CD4 localized?

CD4 is localized in the cell membrane.