Recombinant SARS-CoV-2 (69-70del, Y144del, N501Y, A570D, P681H, T716I, S982A, D1118H Mutant) Stabilized Spike Glycoprotein (Full-Length), His-Strep-tagged

• Cat.No.: S-302S
• Product Overview: SARS-CoV-2 HexaPro stabilized Spike glycoprotein containing deletions and amino acid changes in accordance with variant B.1.1.7 ("UK variant"): 69-70del, Y144del, N501Y, A570D, P681H, T716I, S982A, D1118H relative to Wuhan-Hu-1 with a C-Terminus His-Tag and 2 x Strep-Tag was Expressed in HEK293 and purified by affinity chromatography.
  This protein contains 6 amino acid changes to proline and a C-terminal T4 fibritin trimerization domain to stabilize the protein in its pre-fusion conformation.

Specification

• Description: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes coronavirus disease 2019 (COVID-19). The sequence WIV04/2019, belonging to the GISAID S clade / PANGOLIN A lineage / Nextstrain 19B clade, is believed to be the original sequence infecting humans. However, there are many thousands of variants of SARS-CoV-2 and subtypes of the virus can be placed into much larger groupings such as lineages or clades.
  Lineage B.1.1.7 (Variant of Concern 202012/01) is correlated with a significant increase in the rate of COVID-19 infection in the UK. The two earliest sampled genomes were collected on 20-Sept-2020 in Kent and another on 21-Sept-2020 from Greater London. It was previously known as the first Variant Under Investigation in December 2020 (VUI-202012/01)[20] and also as lineage B.1.1.7 or 20I/501Y.V1 (formerly 20B/501Y.V1). Genomes belonging to lineage B.1.1.7 form a monophyletic clade that is characterized by a large number of lineage-defining mutations. The B.1.1.7 lineage carries a larger than usual number of virus genetic changes with 17 mutations (14 replacements and 3 deletions). It is thought that the unusual genetic divergence of lineage B.1.1.7 may have resulted, at least in part, from virus evolution within a chronically infected individual.
  B.1.1.7 mutations include spike position 501, one of the key contact residues in the receptor binding domain (RBD), and experimental data suggests mutation N501Y can increase ACE2 receptor affinity. N501Y has been associated with increased infectivity and virulence in a mouse model. P681H is a replacement of one of 4 residues comprising the insertion that creates a furin cleavage site between S1 and S2 in spike. The S1/S2 furin cleavage site of SARS-CoV-2 is not found in closely related coronaviruses and has been shown to promote entry into respiratory epithelial cells and transmission in animal models. The spike deletion 69-70del has also occurred a number of times in association with other RBD changes.
• Source: HEK293
• Species: SARS-COV-2
• Tag: His-Strep
• Form: Liquid
• Molecular Mass: Expected Molecular Weight: 139 kDa
  Observed Molecular Weight: 180 kDa
• Protein length: 1-1205
• Purity: Greater than 95% purity.
• Storage: Short Term Storage: -80 centigrade
  Long Term Storage: -80 centigrade
  Can be frozen. Avoid repeated freeze/thaw cycles.
• Concentration: 0.61 mg/mL
• Storage Buffer: DPBS
• Shipping: Dry Ice

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: S
• Synonyms: S; surface glycoprotein; spike glycoprotein; surface glycoprotein; structural protein; spike protein
• Gene ID: 43740568
• mRNA Refseq: MN908947
• Protein Refseq: YP_009724390