Recombinant Treponema Pallidum 15kD Membrane Protein, His-G-tagged

• Cat.No.: TP15-162T

Specification

• Description: Recombinant Treponema pallidum 15Kd protein produced as His-G fusion protein in the E.coli Expression System. Fusion protein protein mol.wt: approx. 42.5kD. This protein binds to murine anti-TP15 Kd protein monoclonal antibodies and Treponema pallidum converted human serum polyclonal antibodies in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.
• Source: E.coli
• Species: Treponema Pallidum
• Tag: His-G
• Form: Frozen Liquid
• Molecular Mass: 42.5 kD.
• Purity: > 95 % by SDS-PAGE
• Applications: In-Vitro Diagnostics;
  Immunization.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
  ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.
• Stability: At least 2 years at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: 25 mM NaPO4, 10mM NaCl, 0.015% Sarcosyl, pH 8