Recombinant Yeast SMT3 Protein, His-tagged

• Cat.No.: SMT3-1244Y
• Product Overview: Recombinant Yeast SMT3 Protein (Met1-Gly97) with His tag was expressed in E. coli.

Specification

• Description: Enables protein tag. Involved in protein sumoylation. Located in condensed nuclear chromosome and septin ring. Used to study cancer. Human ortholog(s) of this gene implicated in orofacial cleft 10 and type 1 diabetes mellitus 5. Orthologous to several human genes including SUMO1 (small ubiquitin like modifier 1).
• Source: E. coli
• Species: Yeast
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.5 kDa; Accurate Molecular Mass: 18 kDa
• Protein length: Met1-Gly97
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: SMT3 SUMO family protein SMT3 [ Saccharomyces cerevisiae S288C ]
• Official Symbol: SMT3
• Synonyms: SMT3; SUMO family protein SMT3; SUMO family protein SMT3; Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant
• Gene ID: 852122
• mRNA Refseq: NM_001180818
• Protein Refseq: NP_010798
• UniProt ID: Q12306