Recombinant Zebrafish ALDOCA

• Cat.No.: ALDOCA-3182Z
• Product Overview: Recombinant Zebrafish ALDOCA full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: aldoca aldolase C, fructose-bisphosphate, a [ Danio rerio (zebrafish) ]
• Official Symbol: ALDOCA
• Gene ID: 792692
• mRNA Refseq: NM_001029952
• Protein Refseq: NP_001025123
• UniProt ID: Q4KMC8

Reviews

01/25/2023

Its versatility and reliability make it an excellent choice for research applications, and I highly recommend it to fellow scientists.

04/18/2021

The ALDOCA Protein's adaptability has significantly expanded the scope of my investigations and broadened the possibilities for my research.

02/23/2021

The sensitivity and specificity of the ALDOCA Protein have consistently delivered robust and consistent results, enhancing the efficiency and accuracy of my experimental workflows.

10/20/2019

It has consistently demonstrated high sensitivity and accuracy in detecting and quantifying target molecules, allowing for precise and reliable measurement of analytes in my experimental samples.

Q&As

Are there any disease associations or clinical implications related to ALDOCA?

ALDOCA-related diseases are rare, but mutations in the ALDOCA gene can cause aldolase A deficiency, a metabolic disorder characterized by skeletal and cardiac muscle weakness, exercise intolerance, and elevated levels of certain metabolites in the blood. Additionally, altered ALDOCA expression or activity has been observed in some cancer types, indicating a potential role in tumorigenesis and progression. However, more studies are necessary to establish a clear understanding of the clinical implications related to ALDOCA.

Can ALDOCA activity be measured or monitored in clinical settings?

Yes, ALDOCA activity can be measured or monitored in clinical settings. One approach is to measure the activity of ALDOCA in blood samples using enzymatic assays. This can provide an indication of ALDOCA deficiency or abnormal glycolytic metabolism in certain metabolic disorders. Additionally, imaging techniques such as positron emission tomography (PET) can be used to assess ALDOCA activity indirectly by measuring glucose uptake and metabolism in tissues, including muscles.

Can ALDOCA expression be regulated by external stimuli?

Yes, ALDOCA expression can be regulated by external stimuli and various signaling pathways. For example, insulin has been shown to increase ALDOCA expression in muscle cells, promoting glucose uptake and utilization. Additionally, certain transcription factors, such as c-Myc and hypoxia-inducible factor 1 (HIF-1), can also influence ALDOCA expression in response to specific environmental conditions.

Can ALDOCA be targeted for therapeutic interventions?

Targeting ALDOCA for therapeutic interventions is an area of active research. In the context of diseases associated with ALDOCA deficiency, such as aldolase A deficiency, gene therapy approaches or enzyme replacement therapy may be explored to restore ALDOCA activity.

Are there any known post-translational modifications of the ALDOCA protein?

Yes, several post-translational modifications have been identified in the ALDOCA protein. These include phosphorylation, acetylation, and sumoylation. Phosphorylation of ALDOCA can modulate its enzymatic activity and subcellular localization, while acetylation and sumoylation have been implicated in regulating its stability and interactions with other proteins.

Are there any disease associations linked to ALDOCA mutations?

Mutations in ALDOCA are not typically associated with genetic diseases. However, alterations in ALDOCA expression have been observed in certain types of cancer. Overexpression of ALDOCA has been reported in various tumors, including breast, lung, and colorectal cancer. These observations suggest a possible role for ALDOCA in cancer development and progression.

Can ALDOCA gene expression be regulated by epigenetic modifications?

Yes, epigenetic modifications, such as DNA methylation and histone modifications, can regulate ALDOCA gene expression. Methylation of the ALDOCA promoter region can lead to gene silencing, reducing the expression of the protein. Additionally, certain histone modifications, such as acetylation or methylation, can also influence ALDOCA expression by altering the accessibility of the gene to transcription factors.

How is ALDOCA protein involved in muscle metabolism?

ALDOCA is highly expressed in muscle tissue and plays a vital role in glucose metabolism. It catalyzes the conversion of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, which are further metabolized to produce energy. ALDOCA deficiency in muscle can impair glycolysis and energy production, potentially leading to muscle weakness or exercise intolerance.

Does ALDOCA have any non-metabolic functions in cells?

While ALDOCA's primary function is related to energy metabolism, emerging research suggests that it may have non-metabolic functions as well. ALDOCA has been implicated in cell signaling pathways, including those involved in cell growth, differentiation, and apoptosis. Some studies have also suggested its potential involvement in cytoskeletal organization and cell migration. However, further research is needed to fully understand the extent and significance of ALDOCA's non-metabolic functions.

Can ALDOCA expression or activity be regulated by dietary factors?

There is limited research on the direct effects of dietary factors on ALDOCA expression or activity. However, studies have shown that certain nutrients, such as glucose, insulin, and amino acids, can influence glycolytic enzyme expression and activity in muscle cells.

Are there any known genetic variations or polymorphisms in the ALDOCA gene?

Yes, genetic variations and polymorphisms have been identified in the ALDOCA gene. These include single nucleotide polymorphisms (SNPs) that can affect the expression or activity of ALDOCA.

Are there any known regulatory factors that control the expression of ALDOCA?

Yes, several regulatory factors have been identified that control the expression of ALDOCA. These include various transcription factors, such as MyoD, MEF2 (myocyte enhancer factor 2), and Sp1 (specificity protein 1), which bind to specific regions of the ALDOCA gene promoter and enhance its transcription. Additionally, insulin signaling and exercise have been shown to positively regulate ALDOCA expression in muscle cells.

What specific functions of ALDOCA are important for muscle metabolism?

ALDOCA plays a critical role in muscle metabolism by catalyzing the reversible conversion of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is necessary for generating ATP (the energy currency of cells) in muscle tissue. ALDOCA also contributes to the regulation of glucose uptake and glycogen synthesis in muscle cells, further supporting muscle energy production.

Are there any known genetic mutations in the ALDOCA gene?

Yes, genetic mutations in the ALDOCA gene have been reported. Some of these mutations are associated with a rare metabolic disorder called aldolase A deficiency, which is characterized by muscle weakness, exercise intolerance, and elevated levels of certain metabolites in the blood. These mutations can affect the stability or catalytic activity of the ALDOCA protein, leading to impaired glycolysis in affected individuals.

Are there any known drugs that target the ALDOCA protein?

Currently, there are no specific drugs available that directly target the ALDOCA protein. However, drugs that target other enzymes involved in glycolysis or energy metabolism may indirectly affect ALDOCA activity. For example, inhibitors of the enzyme hexokinase, which catalyzes the first step of glycolysis, can disrupt the overall glycolytic pathway and subsequently impact ALDOCA function.